They were able to measure the binding of highly specialised protein complexes that specifically change the spatial structure of the genetic information, thereby controlling the readout of the DNA information. The work of Dr. Karsten Rippe and his team was carried out at the BioQuant Center of Heidelberg University and the German Cancer Research Center. Their research has demonstrated that the positioning of nucleosomes – complexes of DNA and specialised proteins – is a precisely regulated molecular process. Aberrant regulation can be linked to several types of cancer. The results of these studies were published in the journal PNAS.
Original publication::F. Erdel, K. Rippe: Quantifying transient binding of ISWI chromatin remodelers in living cells by pixel-wise photobleaching profile evolution analysis, PNAS, 20 November 2012, vol. 109, no. 47, E3221-3230 (online 5 November 2012, doi:10.1073/pnas.1209579109).More information:Dr. Karsten RippeBioQuantUniversity of HeidelbergPhone: +49 (0)6221/ 54 - 51376E-mail: karsten.rippe(at)bioquant.uni-heidelberg.de